Verfahren zum Nachweis der Anwesenheit von Exons 5 und 6 von Anril in einer Person, Kit, Oligonukleotidesets und deren Verwendungen

Details

Veröffentlichungs-Nr.

EP4768604

Anmeldetag

24. Dezember 2024

Veröffentlichung

1. Juli 2026

Rechtsraum

EP

IPC

C12Q1/6886

Abstract

The present invention relates to a method of detecting and/or quantifying the presence of exons 5 and 6 of ANRIL (Antisense Noncoding RNA in the INK4 Locus) in a subject, comprising the following steps of:1) Preparation of total RNA from a biological sample of the subject,2) Conversion of RNA molecules into complementary DNA,3) Loop-mediated isothermal amplification using trans-exonic oligonucleotides that specifically amplify exons 5 and 6,4) Addition of Cas12a/RNA guides complexes, wherein RNA guide specifically targets exons 5 and 6 by base pairing,5) Addition of a reporter DNA probe,6) Detection of cleavage products, wherein detection of cleavage products reveals the presence of exons 5 and 6 of ANRIL, and7) possibly quantifying expression of exons 5 and 6 of ANRIL.The present invention also relates to a method for in vitro diagnosis of a cancer in a subject, to a method for in vitro prognosis a cancer in a subject to a kit for implementing the method and to a set of oligonucleotides.

Anmelder

Firmen

Université de Lorraine
Centre National de la Recherche Scientifique

Land

🇫🇷 Frankreich

Fachgebiete