Verfahren zur Herstellung von 13C-Markierten Biomolekülen

Details

Veröffentlichungs-Nr.

EP4782547

Anmeldetag

28. Januar 2025

Veröffentlichung

29. Juli 2026

Rechtsraum

EP

Abstract

The present invention provides a method for preparation of fully ¹³C isotopically labeled biomolecules or biomolecules having a predetermined ¹³C isotopic labeling by biosynthesis in a mammalian cell line, which comprises the following steps:a) providing a mammalian cell line capable of growing in serum-free media;b) culturing the cell line obtained in step b) in a serum-free medium containing: fully ¹³C isotopically labeled amino acids or ¹³C isotopically labeled amino acids, fully ¹³C isotopically labeled glucose and/or fully ¹³C isotopically labeled galactose, or ¹³C isotopically labeled glucose and/or ¹³C isotopically labeled galactose, fully ¹³C isotopically labeled choline or ¹³C isotopically labeled choline, vitamins, distilled water, insulin, transferrin, sodium selenite, and inorganic salts wherein when the inorganic salt contains carbon, it is preferably fully ¹³C isotopically labeled;c) obtaining ¹³C isotopically labeled biomolecules from the cells or medium after the cultivation of step b).The resulting biomolecules and their mixtures are suitable for use as internal standards in metabolomics and fluxonics, as well as in biotechnological research as standards or markers.

Anmelder

Firma

Univerzita Pardubice

Land

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