Arkray Patente

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An electrochemical sensor (10) includes a base plate (11) provided with a concave part (12) formed on one of surfaces thereof, a fluid channel (13) formed so that a bottom part (12a) of the concave part (12) and the other one of the surfaces of the base plate (11) are communicated with each other, a plurality of electrodes (16 and 17) formed on the concave part; a reagent (19) fixed on the electrodes, a cover (18) which covers the concave part, and an air channel (18a) which causes the inside and outside of the concave part to be communicated with each other.

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A method for quantifying a substance, which method includes the steps of: introducing a sample containing a measurement target substance to a biosensor (B) comprising an enzyme electrode (12) comprising an electrode (12a) and an oxidoreductase (12b) placed on the electrode in a state where direct electron transfer with the electrode occurs, and a counter electrode (13); applying an AC voltage to the enzyme electrode to carry out impedance measurement; and calculating the concentration of the substance based on an index obtained by the impedance measurement.

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A method of driving a pump is used in a pressure-applying apparatus (10), the apparatus including a flow passage (22), a pump (30) configured to impart pressure into the flow passage, an opening and closing valve (26) configured to open and close the flow passage (22), a pressure detector (32) configured to detect pressure in the flow passage (22), and an atmospheric air open valve (34) configured to open an interior of the flow passage (22) to atmospheric air. The method includes driving the pump (30) after closing the opening and closing valve (26) and opening the atmospheric air open valve (34), and evaluating a state of the pump (30), based on one of: the pressure detected by the pressure detector (32) at a time at which a predetermined time period has elapsed after closing the atmospheric air open valve (34), and a time from closing of the atmospheric air open valve (34) until detection of a predetermined pressure by the pressure detector (32).

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A reagent for glutamine synthetase reaction comprising a chelating agent and glutamine synthetase, and a reagent for quantification of ammonia comprising a chelating agent, ATP, glutamic acid, glutamine synthetase, glucose, an oxidized NAD compound, ADP-dependent hexokinase, and glucose-6-phosphate dehydrogenase, are provided.

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The present invention provides a plasma spectrometry method with high reproducibility of plasma light emission. The plasma spectrometry method of the presnet invention includes: a detection step of applying a voltage, thereby detecting plasma light emission; and non-detection step of detecting no plasma light emission. The detection step and the non-detection step are performed repeatedly. In the detection step, a plasma generation step of generating plasma and a plasma non-generation step of generating no plasma are performed alternately. Accordingly, the reproducibility of plasma light emission can be improved.

To provide a cell culture device, a cell culture system and a cell culture method capable of suppressing fluctuations in pressure in a culture fluid, and suppressing gas bubbles from flowing into a cell culture section. A cell culture device including: a cell culture section (12) that cultures cells; a storage section (14) that stores a culture fluid; flow paths (24A) to (24F) that connect the cell culture section (12) and the storage section (14); a fluid delivery device (16) that is provided at the flow paths (24A) to (24F) and that delivers the culture fluid from the storage section (14) to the cell culture section (12); a pressure equalizing unit (18) that is provided at the flow paths (24A) to (24F) and that suppresses fluctuations in pressure imparted to the culture fluid delivered to the cell culture section (12); and a pressurization unit (22) that is provided at the flow path (24E) at a flow outlet side of the cell culture section (12) and that applies a specific pressure to the culture fluid.

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By using a mutant glucose oxidase comprising an amino acid sequence in which a residue corresponding to isoleucine at position 489 or arginine at position 335 in the amino acid sequence of SEQ ID NO: 1 is substituted with an amino acid residue having a reactive functional group in a side chain, and binding an electron acceptor to the mutant glucose oxidase through the amino acid residue having a reactive functional group, an electron acceptor-modified glucose oxidase is obtained.

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A plasma spectroscopy analysis method includes a preliminary addition process in which a bonding agent that is an agent other than DMSA is added to the specimen collected from a living body to which meso-2,3-dimercaptosuccinic acid (DMSA) has been administered, a concentration process in which the analyte heavy metal ions in the specimen at a vicinity of one of a pair of electrodes (20, 30) by applying a voltage to the pair of electrodes (20, 30), and a detection process in which plasma is generated by applying a voltage to the pair of electrodes (20, 30), and luminescence of the analyte metal ions caused by the plasma is detected.

A control metal species that is different from an analyte metal species is added to a sample (60) so as to have a known concentration. The resulting mixture is introduced to a measurement container (10), and an electric current is applied across a pair of electrodes (20, 30) in the measurement container (10) such that the analyte metal species and the control metal species in the sample (60) are concentrated in the vicinity of one of the electrodes (20, 30). An electric current is then applied across the pair of electrodes (20, 30) to generate plasma, and emitted light from the analyte metal species and the control metal species is detected. An analysis emission amount that is a net emission amount at an analysis wavelength that is a wavelength corresponding to the analyte metal species is corrected using a control emission amount that is a net emission amount at a control wavelength that is a wavelength corresponding to the control metal species. The corrected value is compared to a calibration curve obtained by advance measurements of known concentrations of the analyte metal species in order to quantify the analyte metal species in the sample (60).

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A liquid chromatography measurement method includes: switching between a first measurement mode using a liquid chromatography method in which hemoglobin A1c and a hemoglobin variant are measured in a measurement sample by sequentially delivering a first component-separating eluent (A), a second component-separating eluent (C) and a wash eluent (B) to an analytical column (60), and a second measurement mode using the liquid chromatography method in which the hemoglobin A1c is measured by sequentially delivering the first component-separating eluent (A) and the wash eluent (B) to the analytical column (60); delivering the wash eluent (B) in the first measurement mode prior to an influence from the second component-separating eluent (C) disappearing such that a first retention time of the hemoglobin A1c in the first measurement mode and a second retention time of the hemoglobin A1c in the second measurement mode are substantially the same as each other; and delivering the first component-separating eluent (A) after the wash eluent (B).

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