J. Pereira da Cruz, S.A.

The present disclosure refers to a CRISPR/Cas-based gene editing system for conditional activation in human cells, characterized in that the system comprises: a Cas protein or a functionally equivalent variant thereof; and at least one conditionally activatable guide RNA (gRNA), wherein the gRNA is designed to be activated by hybridization with an endogenous intronic RNA molecule in the human cell, thereby enabling targeted DNA cleavage by the Cas protein. It further discloses an in vitro process for activating a guide RNA (gRNA) in a CRISPR/Cas-based gene editing system in human cells, characterized in that the process comprises the following steps: i) providing a conditionally activatable gRNA; ii) exposing the gRNA to an endogenous intronic RNA molecule in the human cell; iii) hybridizing the gRNA with the intronic RNA molecule, thereby activating the gRNA and enabling Cas-mediated DNA cleavage at a target genomic locus, and its use in in vitro targeted gene editing, functional genomics, and therapeutic genome engineering in human cells.

The present disclosure relates to a biogel composition for multitissue regeneration (1), comprising at least one gelling agent (10) configured to form or contribute to the formation of a three-dimensional polymeric network (100), and at least one ionic-modifying agent (20) comprising ferrous ions (Fe²⁺) and/or ferric ions (Fe³⁺), configured to trigger, promote and/or modulate interactions (101) within the biogel composition (1), thereby leading to the formation and/or stabilization of the three-dimensional polymeric network (100), the said three-dimensional polymeric network (100) having viscoelastic behavior. The present disclosure further relates to a system for administering the biogel composition (1), a method for producing a biogel composition (1), and a method for administering the biogel composition (1) onto or within at least one biological surface.

The present invention relates to closure systems for the safe handling of biological samples, specifically for collecting, preserving and transporting procedures.The object of this invention is a closure system for processing biological samples, comprising a first container and a second container configured for fluid storage; a closure assembly comprising a single-piece male cap element and a single-piece female cap element, said male cap element comprising one rupturable membrane; said female cap element comprising a perforation structure configured to rupture said membrane upon actuation; and a locking mechanism configured to prevent the fluid communication between said first and second containers upon rupture of said membrane.The proposed invention aims to reduce the risk of leaks or accidental exposure to harmful vapors and to prevent fixative liquid back flow, while simultaneously ensuring the safety for operators handling hazardous fixatives of biological samples.

The present disclosure relates to a support for securing a LPG bottle during transport or storage (1). In addition to at least one upper face (2) and at least one lower face (3), the support (1) comprises: a concave proximal groove (5) extending transversally from the upper face (2) to a depth configured to accommodate a portion of a neck of an LPG bottle; and a concave distal groove (6) extending transversally from the one upper face (2), longitudinally opposite to the proximal groove (5), to a depth configured to accommodate a portion of a bottom of an LPG bottle; a concave central groove (7) extending transversally from the upper face (2) to a depth configured to accommodate a lateral portion of a body of an LPG bottle; and a through-hole (8) extending transversally between the upper face (2) and the lower face (3) across the central groove (7).

The present disclosure relates to the field of ballistic protection, including materials, structures, and production methods for improving resistance to ballistic threats, impact forces, and penetration. It includes a composite layer (11, 12) for a ballistic armour (10). Such layer may be associated with a ballistic core (20) or with a ballistic core (20) and a hard surface ballistic layer (30), in both cases forming a ballistic armour (10). The composite layer (11, 12) of the present disclosure enables an enhanced and reliable binding to a ballistic core (20) - whichever is the type of ballistic core (20) used - and an enhanced protection to heat.

Provided is a circuit protection device including: a plate-shaped thermistor element comprising a first electrode and a second electrode; a first heat-dissipating terminal connected to the first electrode; a second heat-dissipating terminal connected to the second electrode; and a housing protecting the thermistor element, the first heat-dissipating terminal and the second heat-dissipating terminal. According to the present disclosure, the circuit protection device having a surface mount device (SMD) structure may be applied to a slim wall-mounted television.

It is disclosed a visible light communication system that includes a plurality of emitter units (2), such as LEDs, installed within a communication environment, each capable of emitting data-encoded visible light signals to be received by at least a legitimate receiver unit (3). At least a controller unit (1) is configured to coordinate the operation of the emitter units (2), managing power levels and timing of signals to be transmitted by each emitter unit (2), in order to optimize the data transmission and security.

It is disclosed a method for enhancing security in visible light communication system, by employing constellation decomposition to provide physical layer security. By decomposing and transmitting signal constellations across multiple emitter units (2), that is, across multiple light sources, whose relative positions to a legitimate receiver unit (3) are known, the power and phase delay of each signal constellation are adjusted by a controller unit (1) to ensure that only legitimate receivers (3) are able to accurately reconstruct the transmitted data, thus preventing potential eavesdroppers.

It is an object of the present disclosure a method for generating a cybersecurity test recommendation. Central to the realisation of the test recommendation is the use of a knowledge base model (2) and an inference engine (3) to provide personalised cybersecurity test recommendations (c) to a system (1), based on system (a) and test parameters, in a more accurate and efficient way.

It is disclosed a method for operating a Text-to-Speech (TTS) framework (1) with an integrated Intonation-Aware Subsonic Harmonics (ISH) unit (2, 2.1, 2.2, 2.3, 2.4). Said method including a sequence of steps to generate and integrate inaudible subsonic frequencies (f.) with a synthesized speech waveform (g.), and to dynamically adjust them based on modulation parameters (e.) and contextual feedback (c.) derivable from the text inputted to the TTS system (1). This procedure influences prosodic features results in more natural-sounding, fluid, and expressive synthetic speech signal (h.).