Qiagen

The invention relates to a microfluidic device (100,200,700) for handling a fluid sample by dividing it into a plurality of aliquots, with at least one microfluidic well (105) configured to receive the fluid sample. The at least one microfluidic well comprising an inlet end (120), an outlet end (125) and a microfluidic circuit (110) with a plurality of microchambers (112). The inlet end and the outlet end are respectively coupled to the microfluidic circuit via at least one microfluidic channel (130,130',130",130‴). The plurality of microchambers is arranged such that each microchamber is at least indirectly via further microchambers fluidly coupled with the inlet end and the outlet end such that each microchamber is configured to receive fluid from the inlet end via the at least one microfluidic channel and to provide gas displaced by the fluid downstream to the outlet end. Furthermore, a storage section (140,240,740) with a microfluidic storage circuit (142,242,342,742) is provided in order to store fluid that does not reach and/or flows beyond the microchambers of the microfluidic well during a filling process of the microfluidic device and can therefore not be provided at the intended reaction space, wherein a storage opening structure (144,344) is provided in the storage section.

The invention relates to a microfluidic device (100,200) for handling a fluid sample by dividing it into a plurality of aliquots, with at least one microfluidic well (102) configured to receive the fluid sample. The at least one microfluidic well comprising an inlet end (108), an outlet end (112) and a microfluidic circuit (104) with a plurality of microchambers (106). The inlet end and the outlet end are respectively coupled to the microfluidic circuit via at least one microfluidic channel (110,110',110",110‴). The plurality of microchambers is arranged such that each microchamber is at least indirectly via one or more further microchambers fluidly coupled with the inlet end and the outlet end such that each microchamber is configured to receive sample fluid from the inlet end via the at least one microfluidic channel and to provide gas displaced by the sample fluid downstream to the outlet end. Furthermore, a protective layer (114,214) is applied to the at least one microfluidic well for sealing the inlet end and/or the outlet end of the microfluidic well, wherein the protective layer is at least partially permeable to gas.

The invention relates to a microfluidic device (100, 200, 300) for handling a fluid sample by dividing it into a plurality of aliquots, with at least one microfluidic well (105) configured to receive the fluid sample. The at least one microfluidic well (105) comprising an inlet end (120), an outlet end (125) and a microfluidic circuit (110) with a plurality of microchambers (112). The inlet end and the outlet end are respectively coupled to the microfluidic circuit via at least one microfluidic channel (130, 130', 130", 130‴). The plurality of microchambers is arranged such that each microchamber is at least indirectly via further microchambers fluidly coupled with the inlet end and the outlet end such that each microchamber is configured to receive fluid from the inlet end via the at least one microfluidic channel and to provide gas displaced by the fluid downstream to the outlet end. Furthermore, the at least one microfluidic channel comprises at least one fluidic delay structure (140, 240, 240', 340, 340', 440, 440', 540, 540', 640, 740, 840), which is configured to delay a fluid flow.

The present invention provides methods for quantification of target nucleic acid molecules. In particular, improved digital polymerase chain reaction (dPCR) methods are provided with a broadened dynamic range. Furthermore, the present invention provides systems and computer-readable storage media for said dPCR methods.

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