Waters Technologies Corporation

Aspects of the invention include light detection systems for small particle detection. Light detection systems according to the invention comprise: an optical adjustment component configured to split a beam of light received from a flow cell into first and second beams of light, wherein the first beam of light comprises a light scattered from a particle excited within the flow cell, and wherein the second beam of light comprises a fluorescence of the particle excited within the flow cell; a clustered wavelength division (CWD) light detection subsystem comprising a first light receiver configured to convey the first beam of light to a first light detection module and a second light receiver configured to convey the second beam of light to a second light detection module, wherein the first light detection module comprises a small particle detector; a first optical fiber configured to convey the first beam of light from the optical adjustment component to the first light receiver of the CWD light detection subsystem; and a second optical fiber configured to convey the second beam of light from the optical adjustment component to the second light receiver of the CWD light detection subsystem. In embodiments, the optical adjustment component comprises a beam splitter or a multimodal notch filter. Methods and kits are also provided.

A device for separating analytes is disclosed. The device has a sample injector, sample injection needle, sample reservoir container in communication with the sample injector, chromatography column downstream of the sample injector, and fluid conduits connecting the sample injector and the column. The interior surfaces of the fluid conduits, sample injector, sample reservoir container, and column form a flow path having wetted surfaces. A portion of the wetted surfaces of the flow path are coated with an alkylsilyl coating that is inert to at least one of the analytes. The alkylsilyl coating has the Formula I: <img class="EMIRef" id="37759093-cfc3-4bcf-87b1-737cff9b804e-ia01" /> R<1>, R<2>, R<3>, R<4>, R<5>, and R<6> are each independently selected from (C<1>-C<6>)alkoxy, -NH(C<1>-C<6>)alkyl, -N((C<1>-C<6>)alkyl)<2>, OH, OR<A>, and halo. R<A> represents a point of attachment to the interior surfaces of the fluidic system. At least one of R<1>, R<2>, R<3>, R<4>, R<5>, and R<6> is OR<A>. X is (C<1>-C<20>)alkyl, -O[(CH<2>)<2>O]<1-20>-, -(C<1>-C<10>)[NH(CO)NH(C<1>-C<10>)]<1-20>-, or -(C<1>-C<10>)[alkylphenyl(C<1>-C<10>)alkyl]<1-20>-.

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