Becton Dickinson

Aspects of the present disclosure include systems for classifying cells (e.g., single cells) of a sample in a flow stream such as from biological parameters determined from feature vectors. Systems according to the disclosure include a light source configured to irradiate cells of a sample in a flow stream, a light detection system having a photodetector to detect light from the irradiated cells and a processor having memory operably coupled to the processor where the memory includes instructions stored thereon, which when executed by the processor, cause the processor to receive two or more data sets, where each of the two or more data sets is associated with one of a plurality of data modes, apply an algorithm to convert each of the two or more data sets into a feature vector based on the associated data mode, receive a classification task regarding the two or more data sets and apply one or more of a plurality of classification models to the feature vector based on the received classification task to determine a class. Methods for using the subject systems are also described. Non-transitory computer readable storage media are also provided.

Systems and methods for providing gain-independent flow cytometry data are disclosed. In some implementations, sample median fluorescence intensities (MFIs) may be maintained irrespective of the gain detector settings. In some such implementations, the numerical scale used to represent MFI may correspond to the true intensity of the input signal. As a result, quality control (QC) procedures may instead focus on maintaining resolution performance of the flow cytometer. For example, the QC procedures may instead focus on maximizing a signal-to-noise ratio (SNR) for one or more photodetectors. In some such implementations, the frequency with which such QC procedures are performed may be reduced (e.g., from a daily procedure to a weekly or even monthly procedure). In some such implementations, it may be unnecessary to perform repeated QC procedures to ensure consistent sample MFIs over time.

Aspects of the present disclosure include methods for calibrating spectral data signals (e.g., flow cytometer data). Methods according to certain embodiments include irradiating a particle of a sample having a fluorophore in a flow stream with a light source, detecting light from the irradiated particle with a light detection system having a photodetector, generating spectral data signals in response to the detected light, calibrating the generated spectral data signals based on a calibration scaling factor and a calculated abundance of the fluorophore. Systems and non-transitory computer-readable storage media configured to carry out the subject methods are also provided.

Aspects of the present disclosure include systems for detecting and classifying a cancerous cell in a whole blood sample in a flow stream. Systems according to certain embodiments include a light source configured to irradiate cells of a whole blood sample in a flow stream, a light detection system comprising a photodetector capturing an image of light emitted from the irradiated cells and a processor having memory operably coupled to the processor where the memory includes instructions stored thereon, which when executed by the processor, cause the processor to apply a neural network to the captured image to detect a presence of a cancerous cell in the whole blood sample having a minimum concentration of the cancerous cell and determine a classification of the cancerous cell of a plurality of classifications. In some instances, the neural network is trained using images of a sample tagged with a fluorophore ground truth marker. Methods for using the subject systems to detect and classify cancerous cells in a whole blood sample are also described. Non-transitory computer readable storage media are also provided.

A biological liquid collection device designed to draw blood using an "atmospheric-balanced vacuum" to ensure the blood is exposed to the sample atmospheric partial pressure oxygen and partial pressure carbon dioxide levels as found in standard arterial blood gas syringes, resulting in blood gas sample stabilization during collection and a superior vacuum shelf-life by reducing the gas permeation rate through the plastic tube. The biological liquid collection device comprises a collection module for receiving a biological liquid sample, an evacuated container having an open end and a closed end wherein the evacuated container contains the collection module therein, and a closure for closing the open end of the evacuated container. The evacuated container comprises a gas composition that is substantially equal to the gas composition of the atmosphere outside of the evacuated container. A method for forming the atmospherically balanced vacuum collection device is also provided.

A device for obtaining a blood sample may include a holder for receiving a sample source, the holder having an actuation portion and a port; a blood collector attachment removably connected to the holder; and a collection container removably connected to the blood collector attachment, the container defining a collection cavity, wherein a collection container detachment member is provided between the blood collector attachment and the collection container to releasably attach the collection container to the blood collector attachment.

Flush syringe assemblies are described herein. Such flush syringe assembly may include a barrel including a side wall defining a chamber, a collar mounted on a distal wall of the barrel and surrounding an elongate tip. A cap may be attached to the collar via a hinge. The flush syringe assembly may also include a cap attached to the collar via a hinge. A ring may be disposed around the collar with a locking element that engages with a corresponding mating locking projection on the cap. The cap may include a slanted surface. The slanted surface of the cap may include one or more ribs made of an elastomeric material.

A probe delivery device can include a probe delivery mechanism that facilitates advancing a probe through an IV catheter. The probe delivery mechanism can be housed within a compartment of the probe delivery device and can include a probe that extends from the compartment into a fluid pathway formed within the probe delivery device. A portion of the probe delivery mechanism can extend out from the housing to enable a clinician to advance and retract the probe. The probe delivery device may include a vacuum tube receiver or a connector to enable a blood draw or fluid injection to be performed using the probe delivery device.

Provided herein is a syringe holder syringe holder for enabling measurement of a syringe barrel having a cylindrical main body and a flange positioned at a proximal end of the cylindrical main body. The syringe holder includes at least one rack comprising a top plate including a plurality of top holding apertures, a bottom plate including a plurality of bottom holding apertures, and a plurality of vertical members extending between the top and bottom plates, to space the top plate apart from the bottom plate. Each top holding aperture is aligned with a respective bottom holding aperture, with respective vertically aligned top and bottom holding apertures forming a receptacle configured to receive a syringe barrel therein. A diameter of the top holding aperture of each receptacle in the rack is incrementally different from the diameters of the top holding apertures of the other receptacles.

Aspects of the present disclosure include methods for generating a bitmap from a data plot of light detected from particles in a flow stream. Methods according to certain embodiments include detecting light from particles in a flow stream, generating a data plot of measurements of the detected light, where the data plot includes one or more regions each having a population of particles, calculating a set of vertices that form a boundary for each region in the data plot, identifying a type of algorithmic transformation associated with each vertex in the set of vertices, generating a bitmap of each region of particles such that the bitmap of each region includes a set of vertices that correspond to the vertices of each region in the data plot and identifying an algorithmic transformation for applying to each vertex in the bitmap of each region. Systems and integrated circuit devices (e.g., field programmable gate arrays) having programming for generating a bitmap according to the subject methods are also provided.