K&L Gates LLP

The present disclosure provides for a piston reactor for depositing nanoparticles on structured substrates. According to one aspect of the present disclosure a piston reactor for depositing nanoparticles on structured substrates. According to a second aspect of the present disclosure a method of using a piston reactor for depositing nanoparticles on structured substrates.

The present disclosure provides a universal, high-throughput antibody discovery platform based on: (i) a negative-selection method for enriching B cells or plasma B cells without relying on plasma B cell surface biomarkers, and (ii) a microfluidic droplet-based functional screening system capable of identifying single cells secreting antigen-specific antibodies. The enrichment method employs a customizable cocktail of antibodies that target non-B lineage immune cells, followed by magnetic bead depletion. Enriched antibody-secreting cells are subsequently encapsulated into picoliter or nanoliter droplets together with (a) antigen-coated beads, (b) a fluorescent labeled antigen with immunoglobulin-capture antibody-coated beads, or (c) antigen-expressing reporter cells, allowing for functional detection of secreted antibodies inside each droplet. Through multi-channel fluorescence detection-incorporating detection antibody signals, dead-cell exclusion dyes, and optional blocking-antibody controls-droplets containing antigen-specific antibody-secreting cells are isolated. The recovered cells undergo single-cell BCR amplification or bulk BCR sequencing to obtain naturally paired heavy and light chain sequences.

A method of adding a neural beat having a beat frequency to digital audio comprises: receiving a digital audio file encoding music over a network or from a digital storage device; determining pitch characteristics of the digital audio file at a plurality of timestamps; selecting a time-varying carrier frequency for the neural beat based on the pitch characteristics at the plurality of timestamps; creating a modified version of the digital audio file configured to produce the neural beat having the beat frequency at the time-varying carrier frequency combined with the music when the digital audio file is played; and storing on the digital storage device or transmitting over the network the modified version of the digital audio file.

A control system is provided to receive a plurality of vehicle data from sensors in a vehicle communication network. The control system is disposed on a vehicle in a vehicle system and evaluates a plurality of components of the vehicle system with a predictive model associated with the sensor data. The control system may determine a vehicle status based on the predictive model and modify an operational plan or impose an operational restriction through a propulsion system of the vehicle.

A relay assembly is disclosed herein. The relay assembly can include a first relay with a first reset interface to reset an operating state of the first relay and a second relay with a second reset interface to reset an operating state of the second relay. The second relay can be positioned such that the second reset interface opposes the first reset interface of the first relay. The relay assembly can further include a mechanical armature positioned between the first reset interface and the second reset interface. The mechanical armature can be transitionable between a first position, wherein the mechanical armature is spaced apart from the first reset interface and the second reset interface in the first position, and a second position, wherein the mechanical armature engages the first reset interface and the second reset interface in the second position.

An infant car seat includes a first pair of locking pins positioned and arranged to be locked via a first pair of brackets and a second pair of locking pins positioned and arranged to be locked via a second pair of brackets. The infant car seat further includes a release handle configured to allow a user to unlock the first pair of locking pins from the first pair of brackets- The first pair of brackets and the second pair of brackets are located on a base.

An inspection assistance system includes an insertion unit and a control unit. The control unit acquires an exterior image that is an image of an end portion of each of two or more tubes or an image of a figure indicating the end portion of each of the two or more tubes. The control unit identifies an inspection target in the exterior image based on inspection information and two or more pieces of the identification information corresponding to the two or more tubes in the exterior image. The control unit outputs the exterior image and inspection target information indicating the inspection target to a display before the insertion unit is inserted into one of the two or more tubes or when the insertion unit has been inserted into one of the two or more tubes.

A workgroup-computing-entity-based fail-safe/evolvable hardware core structure is disclosed which includes a 3-hierarchical-level 6-workgroup-Basic-Building-Block (6-wBBB) created to supplant the node-computing-entity-based non-fail-safe/limited evolvable von-Neumann core structure of 3-hierarchical-level 3-node-BBB, (i.e., base-level IO-devices/mid-level main memory/top-level CPU) and all the first-time fail-safe workgroup systems can be subsequently generated in the second period along the workgroup-computing evolutionary timeline. Furthermore, based on the first 6-wBBB evolvable architecture, the workgroup evolutionary processes can go up to 7 generations in creating all the necessary workgroup-computing entity-based hardware core structures, so that all the real-time intelligent workgroup-computing systems can be generated in the third period along the workgroup-computing evolutionary timeline.

A method is disclosed that includes receiving information from an information source and determining whether the received information has information of a type to be encrypted before transmitting the received information to a second communication device. Based at least in part on the determination, the method may include either encrypting the information of the type to be encrypted, generating encrypted information, and transmitting the received information including the encrypted information from the first communication device to the second communication device; or transmitting the received information from the first communication device to the second communication device unencrypted.

A system is provided for connecting a first hose of a first vehicle and a second hose of a second vehicle. The system may include a frame, a lacing actuator, first and second guides coupled to the frame, a first grasper, and a second grasper. The first guide may collect the first hose based on movement of the frame. The second guide may collect the second hose based on movement of the frame. The first grasper may receive the first hose from the first guide and deliver the first hose to the lacing actuator. The second grasper may receive the second hose from the second guide and deliver the second hose to the lacing actuator. The lacing actuator may position a first coupler of the first hose in engagement with a second coupler of the second hose.