Leica Microsystems Patente

A label for analysing a biological sample is provided. The label comprises a first label part comprising a first nucleic acid strand (106) and a second label part comprising a second nucleic acid strand (110). The first nucleic acid strand (106) and the second nucleic acid strand (110) are configured to form a duplex. The label further comprises at least one first labelling moiety (108) and at least one second labelling moiety (112), and the label further comprises at least one blocking nucleic acid strand (126, 128). In further aspects, a marker (100, 500, 600, 700, 1000) comprising the label and a respective method are provided.

A modular capture construct (100, 900, 1000) for capturing a plurality of analytes (402, 504, 604, 706) of a biological sample (1300, 1408) is provided. The modular capture construct (100, 900, 1000) comprises a plurality of modules (102a, 102b, 102c), and at least a first dye (112) and a second dye (114) arranged on the capture construct (100, 900, 1000) to provide an orientation indication. The modular capture construct further comprises at least a first plurality of capture regions (108a, 108b, 108c, 108d, 108e, 108f), each capture region (108a, 108b, 108c, 108d, 108e, 108f) comprising at least one affinity capture reagent (400, 502, 602, 702, 704) configured to capture one of the analytes (402, 504, 604, 706), wherein each module (102a, 102b, 102c) comprises at least one of the capture regions (108a, 108b, 108c, 108d, 108e, 108f) of the first plurality of capture regions (108a, 108b, 108c, 108d, 108e, 108f). Each module (102a, 102b, 102c) comprises a nucleic acid backbone (201). Further, each of the modules (102a, 102b, 102c) is hybridised to at least another one of the modules (102a, 102b, 102c). In a further aspect, a method for detecting a plurality of analytes of a biological sample by means of the modular capture construct is provided.

A first aspect of the present disclosure is related to an imaging device for determining a thickness of a layer comprised in an image,configured to:- obtain an image of a sample with one or more layers ;- obtain from a user interface a first estimation information indicating a location of a first edge of a layer;- obtain from the user interface a second estimation information indicating a location of a second edge of the layer;- determine the first edge of the layer based on the first estimation information;- determine the second edge of the layer based on the second estimation information;- determine a distance between the first edge and the second edge.

A first aspect of the present disclosure is related to a device for image acquisition, configured to: - select a plurality of excitations for exciting a sample, - adjust in a first step: -- a configuration of one of the selected excitations or; -- a configuration of a global excitation , wherein the global excitation is based on one or more of the selected excitations; - adjust in a second step and based on the configuration adjusted in the first step: -- a configuration of one or more of the non-adjusted, selected excitations; and/or -- a configuration of the global excitation, respectively; - providing the adjusted configurations for performing an excitation of the sample.

A controller (142) for a laser microdissection system (100) is configured to receive a user input corresponding to first settings for the laser microdissection system (100) used with a first objective lens (114a), to generate second settings for the laser microdissection system (100) used with a second objective lens (114b) based on the first settings, and to configure the laser micro dissection system (100) based on the second settings. At least one characteristic of a first laser beam generated according to the first settings by the laser microdissection system (100) using the first objective lens (114a) is the same for a second laser beam generated according to the second settings by the laser microdissection system (100) using the second objective lens (114b). The at least one characteristic is influenced by the first objective lens (114a) when the laser microdissection system (100) is used with the first objective lens (114a) and influenced by the second objective lens (114b) when the laser microdissection system (100) is used with the second objective lens (114b).

In a first aspect, a microscope stage (100, 500, 600) is provided comprising a receiving surface (102) configured to receive at least a first sample carrier (104, 200) with a plurality of sample areas (202, 204), an imaging area (206), and a surface moving device (118) configured to move the receiving surface (102) along at least two directions, wherein the surface moving device (118) has a range of movement along the at least two directions such that any one of the sample areas of the plurality of sample areas (202, 204) is alignable with the imaging area (206). The microscope stage further comprises at least one liquid handling head (106, 502, 602) configured to dispense fluids onto the sample carrier (104, 200), and a head moving device (108, 110, 112) configured to move the at least one liquid handling head (106, 502, 602) along at least two directions, wherein the head moving device (108, 110, 112) has a range of movement along the at least two directions such that the at least one liquid handling head (106, 502, 602) is alignable with any one of the sample areas (202, 204) of the plurality of sample areas (202, 204). In further aspects a corresponding microscope and method are provided.

A detection arrangement (102, 302, 402, 602, 704, 806) for an optical scanning microscope (100, 300, 400, 600, 700, 800) comprises a beam splitting element (120) configured to receive descanned detection light (118), to direct a first part (118a) of the detection light (118) having a first wavelength range into a first beam path (128a), and to direct a second part (118b) of the detection light (118) having a second wavelength range different from the first wavelength range into a second beam path (128b). The first beam path (128a) comprises a first array detector (124a) configured to receive the first part (118a) of the detection light (118), and the second beam path (128b) comprises a second array detector (124b) configured to receive the second part (118b) of the detection light (118). The detection arrangement (102, 302, 402, 602, 704, 806) further comprises an upstream dispersive element (126, 804) arranged upstream of the beam splitting element (120) and configured to spectrally separate the descanned detection light (118).

The present disclosure provides an optical system for a lightsheet microscope, the optical system comprising a processor configured to carry out a method for determining a volume of interest, VOI, in a sample, the method comprising processing scanning image data obtained by scanning through the sample with a lightsheet in at least one scanning direction, wherein processing the scanning image data comprises determining borders of an object in the scanning image data along at least two axes; and defining a proposed volume of interest, VOI, based on the determined borders such that the borders along each of the at least two axes are included in the proposed volume of interest, VOI.

In a first aspect, a climate-controlling arrangement (100, 200) for a photodetector unit (104, 202) of an optical system, in particular a microscope system, is provided. The climate-controlling arrangement (100, 200) comprises an enclosure (102) with an optical window (106) and the photodetector unit (104, 202), which is arranged within the enclosure (102). The climate-controlling arrangement (100, 200) further comprises at least one electrolysis unit (108) comprising a membrane (110) arranged in a wall (112) of the enclosure (102). The electrolysis unit (108) is configured to electrolyse water on a surface of the membrane (110) facing an inside (114) of the enclosure (102). In a further aspect, a method for operating the climate-controlling arrangement (100, 200) is provided.

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A light beam characterization system (100) for characterizing a light beam emitted by a light source (102) is disclosed. The light beam characterization system (100) comprises a detector device (108) comprising at least one detector unit (110, 112) configured to detect the light (L), and a micro-opto-electromechanical system (104) comprising an array of mirrors (106). Each mirror (106) is switchable between a first switching state, in which the mirror (106) reflects the light onto the detector device (108), and at least a second switching state, in which the mirror (106) reflects the light (L) away from the detector device (108). A controller (114) is configured to cause a beam profile measurement to be performed on the light (L) detected by the at least one detector unit (110, 112) while selectively switching the mirrors (106) between the first and second switching states. The light beam characterization system (100) further comprises an optical unit (116) configured to direct the light (L) in form of at least two different input light beams (L1, L2) onto the micro-opto-electromechanical system (104). The controller (114) is configured to cause the beam profile measurement to be performed on each of the at least two input light beams (L1, L2).

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The present invention relates to the microscope (100) for examining a sample (112), the microscope (100) comprising a microscope stage (110) for receiving the sample (112) to be examined and further comprising a microscope housing (120) enclosing the microscope stage (110), wherein the microscope housing (120) comprises a first access door (130) and a second access door (240), whereby both, the first access door (130) and the second access door (240), upon opening, provide different ways of access to the microscope stage (110).

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