Université de Lorraine Patente

The present invention relates to a method of detecting the presence of exon 8 of ANRIL (Antisense Non-coding RNA in the INK4 Locus) in a subject, comprising the following steps of:1) Preparation of total RNA from a biological sample of the subject,2) Conversion of RNA molecules into complementary DNA,3) Loop-mediated isothermal amplification using trans-exonic oligonucleotides that specifically produces an amplicon containing exon 8 fragment,4) Addition of Cas12a/RNA guide complexes, wherein RNA guide specifically targets exon 8-containing fragment by base pairing,5) Addition of a reporter DNA probe, and6) Detection of cleavage products,Wherein detection of cleavage products reveals the presence of exon 8 of ANRIL.The present invention also relates to a method for in vitro predicting in vitro the efficacy of a therapy targeting exon 8 of ANRIL in a subject, to a kit for implementing the method and to a set of oligonucleotides.

The present invention relates to a method of extracting glycosylated proteins from dairy stream, said method comprising the step of precipitating the glycosylated proteins of the dairy stream so as to obtain a protein concentrate by mixing a dairy stream with ethanol, thereby obtaining a dairy stream-ethanol solution wherein the ethanol concentration in the dairy stream-ethanol solution is of from 20% to 60% v/v, and wherein the glycoproteins represent at least 30% w/w of the concentrate.

The present invention relates to a method of detecting and/or quantifying the presence of exons 5 and 6 of ANRIL (Antisense Noncoding RNA in the INK4 Locus) in a subject, comprising the following steps of:1) Preparation of total RNA from a biological sample of the subject,2) Conversion of RNA molecules into complementary DNA,3) Loop-mediated isothermal amplification using trans-exonic oligonucleotides that specifically amplify exons 5 and 6,4) Addition of Cas12a/RNA guides complexes, wherein RNA guide specifically targets exons 5 and 6 by base pairing,5) Addition of a reporter DNA probe,6) Detection of cleavage products, wherein detection of cleavage products reveals the presence of exons 5 and 6 of ANRIL, and7) possibly quantifying expression of exons 5 and 6 of ANRIL.The present invention also relates to a method for in vitro diagnosis of a cancer in a subject, to a method for in vitro prognosis a cancer in a subject to a kit for implementing the method and to a set of oligonucleotides.

The present invention relates to a method for producing gamma delta (γδ) T cells. The present invention also relates to the cells obtained by said method and said cells for their use as a medicament and in an immunotherapy, preferably in the treatment or prevention of a cancer.

The present invention relates to a system for preparing a pharmaceutical radioactive hydrogel comprising (i) at least one hydrophilic polymer, particularly at least one polysaccharide derived from chitosan or hyaluronic acid, bearing at least one chelating group and (ii) at least one therapeutical radionuclide chelatable with said chelating group. It further relates to pharmaceutical compositions comprising said hydrophilic polymer and said therapeutical radionuclide chelated with the chelating group Rc of said hydrophilic polymer, their use for treatment of solid cancer, preferably by brachytherapy.

The invention relates to a method for preparing an electrocatalyst comprising a first step of mixing and milling of a metal salt, a polyphenol and a surfactant followed by a second step of heating the paste resulting from the first step at a temperature higher than or equal to 700°C under an N₂ atmosphere.

The invention relates to a method (100) for finding possible configurations of a molecule, comprising iteratively executing an episode comprising: - determining (102) configurations of the molecule from a starting configuration; - determining (106) a reward for each determined configuration, by, at each episode: optimizing (106-2) a target network (TN), from configurations already determined, to achieve a predefined goal, optimizing (106-4) the predictor network (PN), from configurations already determined, to try to replicate an output of the target network (TN), and for each configuration of the current episode, calculating (106-6) the reward from a comparison of the output of the optimized target network (TN) and an output of the optimized predicator network (PN); and - selecting (108) the configuration with the highest novelty reward as the starting configuration for the next episode.

L'invention concerne une structure multicouche (1) pour le refroidissement radiatif passif, comprenant : - une couche réfléchissante (2) de base en argent ou en aluminium, - un empilement (3) déposé sur la couche réfléchissante et comprenant au moins deux et au plus cinq couches émissives (4, 5, 6) superposées les unes aux autres, chaque couche émissive étant continue et d'épaisseur uniforme, au moins une des couches émissives (4) étant en dioxyde d'hafnium, au moins une autre des couches émissives (5, 6) étant en un oxyde, autre que le dioxyde d'hafnium, en un nitrure ou en un oxynitrure, la structure multicouche ayant une épaisseur comprise entre 0,5 et 2 µm.

The invention relates to Redox flow battery comprising at least one electrochemical cell (CEC), a further electrochemical cell (SEC), an electrode disposed or arranged within each electrochemical half-cell, an ion-exchange membrane separating the two electrochemical half-cells of each electrochemical cell and circulating means arranged to circulate a first electrolytic media intended to be received in the battery through an electrochemical half-cell (FCEHC) of each of the at least one electrochemical cell and through an electrochemical half-cell (FSEHC) of the further electrochemical cell. One electrochemical half-cell (SSEHC) of SEC comprises at least one redox couple M^y/M^x insoluble in a second electrolytic media intended to be received in the SSEHC, according to formula 1 M^y + pe^- ↔ M^x, formula 1, wherein x and y are oxidation number. The FSEHC is arranged to reduce an oxidized state C^b to a reduced state C^c, and conversely, of at least one redox couple C^b/C^c comprised in the first electrolytic media according to formula 2 C^b + ne^- ↔ C^c, formula 2, wherein b and c are oxidation number. An absolute potential difference |ΔE₁| between a redox potential E(C^b/C^c) of the at least one couple C^b/C^c, in a given or predetermined first electrolytic media, and a redox potential E(M^y/M^x) of the at least one couple M^y/M^x, in a given or predetermined second electrolytic media, is lower than or equal to 0.8 V.

L'invention concerne un nouveau système d'authentification basé sur l'analyse des spectres de dichroïsme circulaire de molécules biologiques ou organiques qui présentent un taux de diversité extrêmement élevé.

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The disclosure notably relates to a platform (100) for intermittent computing powered by at least one energy source. The platform comprises a non-volatile memory (118). The platform comprises a microcontroller (120) configured for power management of the at least one energy source and for determining a level of available energy. The platform comprises a System-on-Chip (SoC) component comprising a reprogrammable logic (118). The reprogrammable logic comprises an application layer (202) comprising operational blocks implementing one or more applications. The reprogrammable logic comprises an intermittent computing layer (204) configured for executing intermittently the one or more applications of the application layer depending on the determined level of available energy by regularly saving and restoring information from the application layer on the non-volatile memory. Such a platform presents an improved solution of energy-independent and sustainable by design system capable of performing complex processing.

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