Université de Lorraine

The invention relates to a sputtering process for generating a charged particles beam (40), whereby an E x B crossed field is applied to a sputtering target (20) in a vacuum environment, wherein the sputtering target (20) includes a hollow tube-shaped sputtering target (22) extending along a longitudinal axis in which a plasma phase is generated, the target having at least one open end (24, 24'). At least over a predetermined length of the hollow tube-shaped sputtering target (22): - an electric field E is generated inside (27) the hollow tube-shaped sputtering target (22), the electric field gradient being uniform in the vicinity of the sputtering target and normal thereto; and - a first magnetic field B is generated inside (27) the hollow tube-shaped sputtering target (22); According to the invention, the first magnetic field B is generated by means of a first magnet arrangement (30) positioned outside the target (20) and configured to uniformly generate, along said predetermined length, a magnetic field gradient of B whose component Bt is orthoradial to the longitudinal axis, whereby a first unidirectional E x B drift is obtained along the longitudinal axis that induces a linear erosion of the inner surface of the sputtering target along the longitudinal axis as well as an unidirectional and anisotropic linear drift motion of charged particles along the longitudinal axis such that a charged particles beam (40) exits at the open end (24) of the target (20). The invention also relates to a corresponding sputtering device and is suitable in sputtering deposition processes but also in other applications, e.g. implantation, isotopic separation, etching and propulsion.

Device (1) for the separation of right-handed and left-handed chiral nanoparticles (cN) separation comprising a cylindrical Bragg resonator (CBR). The CBR comprises an assembly of dielectric concentric hollow cylinders (CHC) (21, 22, 23, 24) having a same revolution axis (31), a solid dielectric cylinder (SC) (3) coincident with said revolution axis, a volume extending between the inner CHC (24) and said SC (3) forming a duct (32), a solution containing cN being intended to flow through said duct, and two plates (41, 42) extending perpendicularly to said revolution axis; one plate being in contact with one end of each CHC and the other plate being in contact with the other end of each CHC. The assembly of CHC is arranged to support an electromagnetic field (EM) in Transverse Magnetic (TM) modes within the inner CHC, the inner CHC and said two plates form a cylindrical cavity (32, 3) of the CBR, said inner CHC and said two plates being arranged to confine the EM within the cylindrical cavity, the two plates are arranged to generate a constant or uniform electric field (EF1), along the revolution axis, within the inner CHC, and one (42) of said two plates comprises an inlet (421) communicating with said duct and the other plate (41) comprising two radially opposite outlets (411, 412), arranged on both sides of the SC, communicating with said duct, and the device further comprises additional means arranged to generate an additional constant or uniform electric field (EF2), radial to the revolution axis, within the inner CHC.

L'invention concerne un nouveau procédé de cyclisation d'une protéine d'intérêt utilisant une étiquette protéique constituée d'un peptide spécifique (NUFIP1 ou TAH1) et de son récepteur protéique (ZNHIT3 ou PIH1, respectivement) fusionnés respectivement aux extrémités N- et C-terminales de ladite protéine d'intérêt, et la protéine de fusion cyclisée résultante. Cela permet d'augmenter la thermostabilité et/ou d'aider à l'expression de protéines recombinantes et/ou d'aider à la purification de protéines recombinantes d'intérêt rendues plus solubles pour un meilleur rendement de production.

The present invention relates to a method of detecting the presence of exon 8 of ANRIL (Antisense Non-coding RNA in the INK4 Locus) in a subject, comprising the following steps of:1) Preparation of total RNA from a biological sample of the subject,2) Conversion of RNA molecules into complementary DNA,3) Loop-mediated isothermal amplification using trans-exonic oligonucleotides that specifically produces an amplicon containing exon 8 fragment,4) Addition of Cas12a/RNA guide complexes, wherein RNA guide specifically targets exon 8-containing fragment by base pairing,5) Addition of a reporter DNA probe, and6) Detection of cleavage products,Wherein detection of cleavage products reveals the presence of exon 8 of ANRIL.The present invention also relates to a method for in vitro predicting in vitro the efficacy of a therapy targeting exon 8 of ANRIL in a subject, to a kit for implementing the method and to a set of oligonucleotides.

The present invention relates to a method for producing gamma delta (γδ) T cells. The present invention also relates to the cells obtained by said method and said cells for their use as a medicament and in an immunotherapy, preferably in the treatment or prevention of a cancer.

The present invention relates to a method of detecting and/or quantifying the presence of exons 5 and 6 of ANRIL (Antisense Noncoding RNA in the INK4 Locus) in a subject, comprising the following steps of:1) Preparation of total RNA from a biological sample of the subject,2) Conversion of RNA molecules into complementary DNA,3) Loop-mediated isothermal amplification using trans-exonic oligonucleotides that specifically amplify exons 5 and 6,4) Addition of Cas12a/RNA guides complexes, wherein RNA guide specifically targets exons 5 and 6 by base pairing,5) Addition of a reporter DNA probe,6) Detection of cleavage products, wherein detection of cleavage products reveals the presence of exons 5 and 6 of ANRIL, and7) possibly quantifying expression of exons 5 and 6 of ANRIL.The present invention also relates to a method for in vitro diagnosis of a cancer in a subject, to a method for in vitro prognosis a cancer in a subject to a kit for implementing the method and to a set of oligonucleotides.

The present invention relates to a method of extracting glycosylated proteins from dairy stream, said method comprising the step of precipitating the glycosylated proteins of the dairy stream so as to obtain a protein concentrate by mixing a dairy stream with ethanol, thereby obtaining a dairy stream-ethanol solution wherein the ethanol concentration in the dairy stream-ethanol solution is of from 20% to 60% v/v, and wherein the glycoproteins represent at least 30% w/w of the concentrate.

The present invention relates to a system for preparing a pharmaceutical radioactive hydrogel comprising (i) at least one hydrophilic polymer, particularly at least one polysaccharide derived from chitosan or hyaluronic acid, bearing at least one chelating group and (ii) at least one therapeutical radionuclide chelatable with said chelating group. It further relates to pharmaceutical compositions comprising said hydrophilic polymer and said therapeutical radionuclide chelated with the chelating group Rc of said hydrophilic polymer, their use for treatment of solid cancer, preferably by brachytherapy.

The invention relates to a method for preparing an electrocatalyst comprising a first step of mixing and milling of a metal salt, a polyphenol and a surfactant followed by a second step of heating the paste resulting from the first step at a temperature higher than or equal to 700°C under an N₂ atmosphere.

The invention relates to a method (100) for finding possible configurations of a molecule, comprising iteratively executing an episode comprising: - determining (102) configurations of the molecule from a starting configuration; - determining (106) a reward for each determined configuration, by, at each episode: optimizing (106-2) a target network (TN), from configurations already determined, to achieve a predefined goal, optimizing (106-4) the predictor network (PN), from configurations already determined, to try to replicate an output of the target network (TN), and for each configuration of the current episode, calculating (106-6) the reward from a comparison of the output of the optimized target network (TN) and an output of the optimized predicator network (PN); and - selecting (108) the configuration with the highest novelty reward as the starting configuration for the next episode.